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Method For Preparing Genome Library, And Genome Library Prepared By The Method

机译:基因组文库的制备方法,以及用该方法制备的基因组文库

摘要

There is provided a first method of preparing a genome library in which PCR is carried out with the use of genomic DNA of target biological species per se or fragment thereof as a direct template and with the use of random primer or one type of primer of specified sequence so as to effect genome amplification, thereby obtaining a genuine library. There is further provided a second method of preparing a genome library in which genome of target biological species is pretreated and thereafter PCR is carried out with the use of one type of primer of inherent sequence so as to effect genome amplification, thereby obtaining a genome library. In both of the methods, a genome library can be prepared conveniently from a minute amount of sample.
机译:提供了制备基因组文库的第一种方法,其中使用靶标生物物种本身的基因组DNA或其片段作为直接模板并使用随机引物或一种指定的引物进行PCR序列以实现基因组扩增,从而获得真正的文库。还提供了第二种制备基因组文库的方法,其中,对目标生物物种的基因组进行预处理,然后使用一种固有序列的引物进行PCR以实现基因组扩增,从而获得基因组文库。 。在这两种方法中,都可以方便地从微量样品中制备基因组文库。

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