首页>外文期刊>生物科技>分子生物学>Current Protocols in Molecular Biology

Current Protocols in Molecular Biology

MEDLINE

中文名称：当前的分子生物学技术

ISSN：1934-3639
出版周期：Quarterly,

发文量：92

期刊论文

热门论文

年度选择

更多>>

全选（0）

1.Using Barcoded HIV Ensembles (B-HIVE) for Single Provirus Transcriptomics

机译：使用条形为HIV系列（B-HIVE）用于单个潜意症转录组织
- 作者：Heng-Chang Chen;Eduard Zorita;Guillaume J. Filion
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第122期
- B-HIVE;
- HIV;
- latency;
- molecular barcodes;
- single-provirus expression;
2.Digital Droplet PCR for Monitoring Tissue-Specific Cell Death Using DNA Methylation Patterns of Circulating Cell-Free DNA

机译：用于监测组织特异性细胞死亡的数字液滴PCR使用循环无细胞DNA的DNA甲基化模式
- 作者：Ruth Shemer;Judith Magenheim;Yuval Dor
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- bisulfite;
- blood test;
- cell-free DNA;
- cfDNA;
- ddPCR;
- DNA methylation;
3.In Situ Hybridization for Detecting Mature MicroRNAs In Vivo at Single-Cell Resolution

机译：用于在单细胞分辨率下检测体内成熟微小RNA的原位杂交
- 作者：Ama L. Minogue Swathi Arur
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- C. elegans;
- in situ hybridization;
- locked nucleic acid probe;
- microRNA;
- oogenesis;
4.Polysome Profiling Analysis of mRNA and Associated Proteins Engaged in Translation

机译：mRNA和相关蛋白的多血细胞分析分析从事翻译
- 作者：Eric S. Pringle;Craig McCormick;Zhenyu Cheng
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- efficiency;
- mRNA;
- ribosome;
- translation;
- translational;
- polysomes;
5.CRISPR-Cas9-Guided Genome Engineering in Caenorhabditis elegans

机译：Carpr-Cas9引导的基因组工程在Caenorhabditis elegans
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- C. elegans;
- Cas9;
- CRISPR;
- CRISPR-Cas;
- genome editing;
- genome engineering;
6.Low-Input MNase Accessibility of Chromatin (Low-Input MACC)

机译：染色质的低输入Mnase可访问性（低输入MACC）
- 作者：Mattia Lion;Michael Y. Tolstorukov;Marjorie A. Oettinger
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- accessibility;
- chromatin;
- low-cell-number;
- MNase;
7.deltaTE: Detection of Translationally Regulated Genes by Integrative Analysis of Ribo-seq and RNA-seq Data

机译：漂白：通过RIBO-SEQ和RNA-SEQ数据的整合分析检测翻译规则基因
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- deltaTE;
- Ribo-seq;
- RNA-seq;
- translation efficiency;
- translational regulation;
8.MapR: A Method for Identifying Native R‐Loops Genome Wide

机译：Mapr：一种识别本机R-LOPS Genome广泛的方法
- 作者：Yan Qingqing;Sarma Kavitha
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第130期
- chromatin;
- DNA:RNA hybrids;
- gene expression;
- R‐loops;
- transcription;
9.Generating Single Cell-Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9

机译：在哺乳动物细胞中生成单细胞衍生的敲除克隆，用CRISPR / CAS9
- 作者：Christopher J. Giuliano
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
10.The Use of the Fluidigm C1 for RNA Expression Analyses of Single Cells

机译：使用流体C1进行单细胞的RNA表达分析
- 作者：Daniel M. DeLaughter
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第122期
- Fluidigm C1;
- RNA-seq;
- single-cell;
11.Multigene Engineering by GoldenBraid Cloning: From Plants to Filamentous Fungi and Beyond

机译：Muldbbraid Cloning的多岛工程：从植物到丝状真菌和超越
- 作者：Vazquez‐Vilar Marta;Gandía Mónica;García‐Carpintero Victor;Marqués Eric;Sarrion‐Perdigones Alejandro;Yenush Lynne;Polaina Julio;Manzanares Paloma;Marcos Jose F.;Orzaez Diego
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第130期
- Agrobacterium‐mediated transformation;
- FungalBraid;
- GoldenBraid;
- modular cloning;
- synthetic biology;
12.Rapid Tagging of Human Proteins with Fluorescent Reporters by Genome Engineering using Double-Stranded DNA Donors

机译：使用双链DNA供体，基因组工程快速标记用荧光报告者用荧光报道者的蛋白质
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- Cas9;
- CRISPR;
- double-stranded DNA donors;
- fluorescent protein;
- genome engineering;
- homology-directed repair;
- tissue culture cells;
13.Targeted Profiling of RNA Translation

机译：RNA翻译的有针对性的分析
- 作者：Ben B. Li;Changli Qian;Thomas M. Roberts;Jean J. Zhao
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- protein synthesis;
- RNA translation;
- RT-qPCR;
14.Targeted Transcriptional Activation in Plants Using a Potent Dead Cas9-Derived Synthetic Gene Activator

机译：使用效率死亡Cas9衍生的合成基因活化剂靶向植物的转录激活
- 作者：Zhenxiang Li;Fengzhu Wang;Jian-Feng Li
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- CRISPR;
- dCas9 gene activator;
- plants;
- promoter;
- transcriptional activation;
15.Human Induced Pluripotent Stem Cell Production and Expansion from Blood using a Non-Integrating Viral Reprogramming Vector

机译：使用非整合病毒重编程载体，人诱导多能干细胞产生和扩张
- 作者：Arun Sharma;Michael Mucke;Christine E. Seidman
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第122期
- cell fate;
- iPSCs;
- PBMC;
- pluripotent stem cells;
- reprogramming;
- Sendai virus;
16.Rfoot: Transcriptome-Scale Identification of RN A-Protein Complexes from Ribosome Profiling Data

机译：Rfoot：来自核糖体分析数据的RN A-蛋白复合物的转录组尺度鉴定
- 作者：Zhe Ji
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第124期
- lncRNA;
- non-ribosomal protein-RNA complex;
- ribosome profiling;
- RNase footprinting;
- small noncoding RNA;
17.Somatic Liver Knockout (SLiK): A Quick and Efficient Way to Generate Liver‐Specific Knockout Mice Using Multiplex CRISPR/Cas9 Gene Editing

机译：躯体肝敲除（Slik）：使用多路复用CRISPR / CAS9基因编辑产生肝细胞特异性敲除小鼠的快速有效的方法
- 作者：Johnson Collin G.;Chen Tong;Furey Nika;Hemmingsen Madeline G.;Bissig Karl‐Dimiter
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第130期
- animal model;
- CRISPR;
- genome engineering;
- somatic liver knockout;
18.Production of Purified CasRNPs for Efficacious Genome Editing

机译：生产纯化的CasRNPS，用于有效的基因组编辑
- 作者：Emily Lingeman;Chris Jeans;Jacob E. Corn
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第120期
- Cas9;
- genome editing;
- ribonucleoprotein;
- RNP;
- CRISPR;
19.CASAAV: A CRISPR-Based Platform for Rapid Dissection of Gene Function In Vivo

机译：Casaav：基于CRISPR的平台，用于在体内快速解剖基因功能
- 作者：Nathan J. VanDusen;Yuxuan Guo;Weiliang Gu;William T. Pu
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第120期
- CRISPR;
- Adeno-associated virus;
- Mouse model;
- Genetic mosaic;
- Cardiomyocyte;
20.Optimizing Tissue Preservation for High-Resolution Confocal Imaging of Single-Molecule RNA-FISH

机译：优化单分子RNA鱼的高分辨率共聚焦成像组织保存
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- alcohol fixation;
- formalin fixation;
- RNA degradation;
- singlemolecule RNA-FISH;
- tissue preservation;
21.T7 Expression Systems for Inducible Production of Proteins from Cloned Genes in E. coli

机译：T7表达系统，用于克隆基因在大肠杆菌中克隆基因的诱导蛋白
- 作者：F. William Studier
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第124期
- asymmetric ligation;
- auto-induction;
- basal expression;
- co-expression;
- MDAG non-inducing growth media;
- BL21(DE3);
- BL21-AI;
- pAL vectors;
22.Synthetic DNA Assembly Using Golden Gate Cloning and the Hierarchical Modular Cloning Pipeline

机译：使用金门克隆的合成DNA组件和分层模块化克隆管道
- 作者：Marillonnet Sylvestre;Grützner Ramona
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第130期
- DNA assembly;
- DNA construction;
- Golden Gate cloning;
- MoClo;
- modular cloning;
- multigene constructs;
- synthetic biology;
23.Two-Step Co-Immunoprecipitation (TIP)

机译：两步共免疫沉淀（尖端）
- 作者：Maria Rita Sciuto;Valeria Coppola;Gioacchin Iannolo;Ruggero De Maria;Tobias L. Haas
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- antibody;
- co-immunoprecipitation;
- protein-nucleic acid interaction;
- protein-protein interaction;
24.A Detailed Protocol for Subcellular RNA Sequencing (subRNA-seq)

机译：亚细胞RNA测序的详细方案（Subrna-SEQ）
- 作者：Andreas Mayer L. Stirling Churchman
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第120期
- Cell fractionation;
- subcellular RNA-seq;
- nascent RNA;
- RNA polymerase II (Pol II);
- transcription;
- next-generation sequencing;
- RNA processing;
25.STARR-seq and UMI-STARR-seq: Assessing Enhancer Activities for Genome-Wide-, High-, and Low-Complexity Candidate Libraries

机译：STARR-SEQ和UMI-STAR-SEQ：评估基因组，高，低复杂性候选图书馆的增强剂活动
- 作者：Christoph Neumayr;Michaela Pagani
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
26.Overview of Next-Generation Sequencing Technologies

机译：下一代测序技术概述
- 作者：Barton E. Slatko;Andrew F. Gardner;Frederick M. Ausubel
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第122期
- next-generation sequencing;
- NGS;
- Sanger sequencing;
27.Whole Genome Next-Generation Sequencing Mutation Identification in Pseudomonas aeruginosa

机译：Pseudomonas铜绿假单胞菌的全基因组下一代测序突变鉴定
- 作者：Murat Cetinbas;Shen Yu;Ruslan I. Sadreyev
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第124期
28.RibORF: Identifying Genome-Wide Translated Open Reading Frames Using Ribosome Profiling

机译：Riborf：使用核糖体分析识别基因组翻译的开放阅读框架
- 作者：Zhe Ji
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第124期
- noncoding RNA;
- open reading frame;
- ribosome profiling;
- translation;
29.Single-Cell RNA-seq: Introduction to Bioinformatics Analysis

机译：单细胞RNA-SEQ：生物信息学分析简介
- 作者：Fei Ji Ruslan I. Sadreyev
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- bioinformatics;
- single-cell RNA-seq;
30.Chromatin-Associated RNA Sequencing (ChAR-seq)

机译：染色质相关的RNA测序（Char-SEQ）
- 作者：David Jukam;Charles Limouse;Owen K. Smith;Viviana I. Risca;Jason C. Bell
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- chromatin;
- lncRNA;
- noncoding RNA;
- proximity ligation;
- RNA;
- RNA-DNA interactome;
- ChAR-seq;
31.Pooled CRISPR Screens in Drosophila Cells

机译：在果蝇细胞中汇集Crispr屏幕
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- CRISPR;
- Drosophila;
- functional genomics;
- high-throughput screening;
- pooled-format screening;
32.Growth of E. coli in Liquid Medium

机译：液体介质中大肠杆菌的生长
- 作者：Karen L. Elbing Roger Brent
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- aeration;
- cell counting;
- Escherichia coli K-12;
- hemacytometer;
- spectrophotometer;
33.Plate Design for and Cherry Picking of Bacterial RNAi Clones for Systematic Error Detection in High-Throughput Caenorhabditis elegans RNAi Screens

机译：细菌RNAi克隆的板设计和樱桃挑选，用于系统误差检测高通量caenorhabditis elegans rnai屏幕
- 作者：Sakthimala Jagadeesan Abdul Hakkim
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第124期
- automated high-throughput screening;
- C. elegans;
- reverse genetics;
- RNAi;
- RNAi feeding;
- RNAi library design;
34.Amplification Techniques for the Detection of Misfolded Prion Proteins in Experimental and Clinical Samples

机译：用于检测实验和临床样品中错折叠朊病毒蛋白的扩增技术
- 作者：Haley Nicholas
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第130期
- amplification;
- PMCA;
- prion;
- RT‐QuIC;
- chronic wasting disease;
- Creutzfeldt‐Jakob disease;
35.An Effective Recombinant Protein Expression and Purification System in Saccharomyces cerevisiae

机译：酿酒酵母中有效的重组蛋白表达和纯化系统
- 作者：Ying Xie;Xiao Han;Yansong Miao
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第123期
- actin filament;
- budding yeast;
- protein purification;
- post-translational modification;
- recombinant protein expression;
36.High-Resolution Chromatin Profiling Using CUT&RUN

机译：使用切割和运行的高分辨率染色质分析
- 作者：Sarah J. Hairier Thomas G. Fazzio
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- chromatin;
- CUTamp;
- RUN;
- protein A-micrococcal nuclease;
- transcription factor;
37.RNAi Screening: Automated High-Throughput Liquid RNAi Screening in Caenorhabditis elegans

机译：RNAi筛选：胶巢杆菌中的自动化高通量液体RNAi筛选
- 作者：Sakthimala Jagadeesan Abdul Hakkim
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第124期
- automated high-throughput screening;
- C. elegans;
- reverse genetics;
- RNAi;
- RNAi feeding;
38.Use of the CRISPR‐Cas9 System in Drosophila Drosophila Cultured Cells to Introduce Fluorescent Tags into Endogenous Genes

机译：使用CRISPR-CAS9系统在果蝇果蝇培养细胞将荧光标签引入内源基因
- 作者：Bosch Justin A.;Knight Shannon;Kanca Oguz;Zirin Jonathan;Yang‐Zhou Donghui;Hu Yanhui;Rodiger Jonathan;Amador Gabriel;Bellen Hugo J.;Perrimon Norbert;Mohr Stephanie E.
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第130期
- cell culture;
- CRISPaint;
- CRISPR;
- Drosophila;
- fluorescent protein tagging;
- gene tagging;
- GFP fusion;
- knock‐in;
- ssDNA Drop‐In;
39.Making Use of Cancer Genomic Databases

机译：利用癌症基因组数据库
- 作者：Chad J. Creighton
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第121期
40.Avian Retrovirus-Mediated Tumor-Specific Gene Knockout

机译：禽逆转录病毒介导的肿瘤特异性基因敲除
- 作者：Wei Wang;Bingning Dong;Feng Yang
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第121期
41.Transfection by Electroporation

机译：通过电穿孔进行转染
- 作者：Huntington Potter Richard Heller
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第121期
42.CRISPR-Cas9-Edited Site Sequencing (CRES-Seq): An Efficient and High-Throughput Method for the Selection of CRISPR-Cas9-Edited Clones

机译：CRISPR-CAS9编辑的站点测序（CRES-SEQ）：选择CRISPR-CAS9编辑克隆的有效和高通量方法
- 作者：Yaligara Veeranagouda;Delphine Debono-Lagneaux;Hamida Fournet;Gilbert Thill Michel Didier
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第121期
43.Pooled Lentiviral-Delivery Genetic Screens

机译：合并的慢病毒 - 递送遗传筛
- 作者：Federica Piccioni;Scott T. Younger;David E. Root
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第121期
44.CRISPR/Cas9-Mediated Genome Editing in Epstein-Barr Virus-Transformed Lymphoblastoid B-Cell Lines

机译：CRISPR / CAS9介导的Epstein-Barr病毒转化淋巴细胞B细胞系的基因组编辑
- 作者：Sizun Jiang;Liang Wei Wang;Michael J. Walsh;Stephen J. Trudeau
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第121期
45.Modulating Gene Expression in Epstein-Barr Virus (EBV)-Positive B Cell Lines with CRISPRa and CRISPRi

机译：用Crispra和Crispri调节Epstein-Barr病毒（EBV）阳性B细胞系中的基因表达
- 作者：Liang Wei Wang;Stephen J. Trudeau;Chong Wang;Catherine Gerdt;Sizun Jiang;Bo Zhao
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第121期
46.Differential Radial Capillary Action of Ligand Assay (DRaCALA)

机译：配体测定的差分径向毛细管作用（Dracala）
- 作者：Anna B. Seminara;Asan Turdiev;Husan Turdiev;Vincent T. Lee
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
47.Single-Assay Profiling of Nucleosome Occupancy and Chromatin Accessibility

机译：核小体占用和染色质可达性的单鉴定分析
- 作者：April Cook;Jakub Mieczkowski;Michael Y. Tolstorukov
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第120期
- chromatin;
- nucleosomes;
- accessibility;
- MNase titration;
48.Expression and Purification of Recombinant Proteins Using the Baculovirus System

机译：使用杆状病毒系统的重组蛋白的表达和纯化
- 作者：Cheryl Isaac Murphy;Helen Piwnica-Worms;Stefan Griinwald;William G. Romanow;Nicole Francis;Hua-Ying Fan;Sharon Marr
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第123期
- baculovirus;
- expression;
- purification;
- recombinant protein;
49.How to Perform miRacles: A Step‐by‐Step microRNA Detection Protocol Using DNA Nanoswitches

机译：如何执行奇迹：使用DNA纳米开关的一步一步的MicroRNA检测协议
- 作者：Chandrasekaran Arun Richard;Dey Bijan K.;Halvorsen Ken
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第130期
- biosensing;
- DNA nanoswitch;
- DNA nanotechnology;
- microRNA detection;
- miRacles;
- Northern blotting;
- qPCR;
- total RNA;
50.Growth of E. coli on Solid Media

机译：固体介质中大肠杆菌的生长
- 作者：Karen L. Elbing Roger Brent
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- agar plates;
- petri dish;
- spreading;
- stab vial;
- streaking;
51.Higher Mass Accuracy MALDI-TOF/TOF Lipid Imaging of Human Brain Tissue in Alzheimer's Disease

机译：阿尔茨海默病中人脑组织的较高质量准确度MALDI-TOF / TOF脂质成像
- 作者：Matthew B. ORourke;Caine C. Smith;Suzanne M. De La Monte;Greg T. Sutherl;Matthew P. Padula
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- IMS;
- lipid imaging;
- MALDI imaging;
- sublimation;
52.Protein Binding to mRNA 3' Isoforms

机译：蛋白质与mRNA 3'同种型结合
- 作者：Joseph V. Geisberg Zarmik Moqtaderi
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
53.Simple CRISPR-Cas9 Genome Editing in Saccharomyces cerevisiae

机译：Saccharomyces Cerevisiae中简单的CRAP-CAS9基因组编辑
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
- Cas9;
- CRISPR;
- genome editing;
- vectors;
- yeast;
54.Introduction to Single-Cell RNA Sequencing

机译：单细胞RNA测序介绍
- 作者：Thale Kristin Olsen;Ninib Baryawno
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第122期
- gene expression profiling;
- RNA sequencing;
- single-cell analysis;
55.Transposon Insertion Site Sequencing (TIS-Seq): An Efficient and High-Throughput Method for Determining Transposon Insertion Site(s) and Their Relative Abundances in a PiggyBac Transposon Mutant Pool by Next-Generation Sequencing

机译：转座子插入位点测序（TIS-SEQ）：通过下一代测序确定转座子插入位点的高效和高通量方法，用于测定储纸转座子突变体池中的转座插入位点及其相对丰度
- 作者：Yaligara Veeranagouda1 Michel Didier1
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第120期
- PiggyBac;
- transposon insertion;
- transposon mutant;
- high throughput;
- mutant screening;
56.Probing In Vivo Structure of Individual mRNA 3' Isoforms Using Dimethyl Sulfate

机译：使用二甲基硫酸甲酯的单个mRNA 3'同种型体内结构探测
- 作者：Zarmik Moqtaderi Joseph V. Geisberg
- 期刊名称：《Current Protocols in Molecular Biology》 | 2019年第2019期
57.DMS-seq for In Vivo Genome-Wide Mapping of Protein-DNA Interactions and Nucleosome Centers

机译：DMS-SEQ用于蛋白质-DNA相互作用和核心为中心的体内基因组映射
- 作者：Taichi Umeyama;Takashi Ito
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第123期
- DNA-binding protein;
- epigenome;
- genomic footprinting;
- chromatin;
58.RNA-seq: Basic Bioinformatics Analysis

机译：RNA-SEQ：基础生物信息学分析
- 作者：Fei Ji1;Ruslan I. Sadreyev
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第124期
- bioinformatics;
- differentially expressed genes;
- quantitative analysis of gene expression;
- RNA-seq;
59.CRISPR/Cas9-Based Multiplex Genome Editing in Monocot and Dicot Plants

机译：单子棉和床植物中基于CRISPR / CAS9的多重基因组编辑
- 作者：Xingliang Ma;Yao-Guang Liu
- 期刊名称：《Current Protocols in Molecular Biology》 | 2016年第116期
- CRISPR/Cas9;
- multiplex genome editing;
- mutant genotyping;
- plants;
60.ARF-AID: A Rapidly Inducible Protein Degradation System That Preserves Basal Endogenous Protein Levels

机译：ARF-AID：一种快速诱导的蛋白质降解系统，可保留基础内源蛋白水平
- 作者：Kizhakke Mattada Sathyan;Thomas G. Scott;Michael J. Guertin
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第132期
- ARF-AID;
- auxin;
- auxin-inducible degron;
- protein degradation;
61.Strand-Specific Transcriptome Sequencing Using SMART Technology

机译：使用智能技术的链特异性转录组测序
- 作者：Magnolia Bostick;Nathalie Bolduc;Alisa Lehman;Andrew Farmer
- 期刊名称：《Current Protocols in Molecular Biology》 | 2016年第116期
- stranded RNA-seq;
- rRNA removal;
- non-coding RNA;
- degraded RNA;
62.Virion Display: A High-Throughput Method to Express Functional Membrane Proteins

机译：病毒赛：表达功能膜蛋白的高通量方法
- 作者：Guan-Da Syu;Eric Johansen;Heng Zhu
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第132期
- HSV-1;
- membrane protein;
- transmembrane;
- virion display;
63.Transfection by Electroporation

机译：通过电穿孔进行转染
- 作者：Huntington Potter Richard Heller
- 期刊名称：《Current Protocols in Molecular Biology》 | 2018年第121期
64.Universal Precautions: Necessary Safety Procedures When Handling Human Blood, Body Fluids, and Specimens

机译：通用预防措施：处理人类血液，体液和标本时所需的安全程序
- 作者：Barbara E. Bierer
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第118期
- universal precautions;
- blood;
- body fluid;
- safety;
65.Detection of Cell Proliferation Markers by Immunofluorescence Staining and Microscopy Imaging in Paraffin-Embedded Tissue Sections

机译：通过免疫荧光染色和显微镜成像在石蜡包埋组织切片中的检测方法
- 作者：Seda Eminaga;Polakit Teekakirikul;Christine E. Seidman;Jonathan G. Seidman
- 期刊名称：《Current Protocols in Molecular Biology》 | 2016年第116期
- BrdU;
- cell proliferation;
- immunofluorescence;
- immunohistochemistry;
- Ki67;
66.Immunization of Mice

机译：小鼠的免疫
- 作者：Tomas Maira-Litran
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第117期
- adjuvant;
- antigen;
- monoclonal antibody;
67.High-Throughput Cellular RNA Sequencing (HiCAR-Seq): Cost-Effective, High-Throughput 3' mRNA-Seq Method Enabling Individual Sample Quality Control

机译：高通量蜂窝RNA测序（HICAR-SEQ）：成本效益，高通量3'mRNA-SEQ方法，使单独的样本质量控制
- 作者：Yaligara Veeranagouda;Jean-Luc Zachayus;Jean-Claude Guillemot;Olivier Venier;Michel Didier
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第132期
- 3' mRNA-Seq;
- drug screening;
- gene expression;
- high throughput;
- RNA-Seq;
- sample quality control;
68.Reconstitution and Purification of Nucleosomes with Recombinant Histones and Purified DNA

机译：重组组蛋白和纯化DNA重建和纯化核体
- 作者：Ilana M. Nodelman;Ashok Patel;Robert F. Levendosky;Gregory D. Bowman
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第133期
- Bio-Rad Prep Cell/Mini Prep Cell;
- DNA purification;
- large-scale PCR;
- nucleosome purification;
- nucleosome reconstitution;
69.Proximity-CLIP and Expedited Non-Radioactive Library Preparation of Small RNA Footprints for Next-Generation Sequencing

机译：接近剪辑，加快非放射性库的小RNA占地面积为下一代测序
- 作者：Dimitrios Anastasakis;Daniel Benhalevy;Markus Hafner
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第131期
70.Simultaneous Examination of Cellular Pathways using Multiplex Hextuple Luciferase Assaying

机译：使用多重HExtuple荧光素酶测定同时检查细胞途径
- 作者：Alejro Sarrion-Perdigones;Lyra Chang;Yezabel Gonzalez;Tatiana Gallego-Flores;Damian W. Young;Koen J.T. Venken
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第131期
- assay;
- cell culture;
- cellular signaling pathway;
- hextuple;
- high-throughput;
- luciferase;
- multiplex;
- orthogonal;
- pathway perturbation;
- plate reader;
- transfection;
71.Base Editing in Human Cells to Produce Single-Nucleotide-Variant Clonal Cell Lines

机译：在人体细胞中进行基础编辑以产生单核苷酸变异克隆细胞系
- 作者：Carlos A. Vasquez;Quinn T. Cowan;Alexis C. Komor
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第133期
- base editing;
- genome editing;
- isogenic cell lines;
- single-nucleotide variant;
72.Production of F(ab')2 from Monoclonal and Polyclonal Antibodies

机译：从单克隆和多克隆抗体产生f（ab'）2
- 作者：Shai Rosenstein;Anna Vaisman-Mentesh;Limor Levy;Aya Kigel;Yael Dror;Yariv Wine1
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第131期
- IdeS;
- mAbs;
- monoclonal antibody;
- polyclonal antibodies;
73.Measuring Protein Synthesis in Cultured Cells and Mouse Tissues Using the Non-radioactive SUnSET Assay

机译：使用非放射性日落测定测量培养细胞和小鼠组织中的蛋白质合成
- 作者：Venkatraman Ravi;Aditi Jain;Sneha Mishra;Nagalingam Ravi Sundaresan
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第133期
- cell culture;
- mouse;
- protein synthesis;
- puromycin;
- SUnSET assay;
74.Analytical Ultracentrifugation (AUC): An Overview of the Application of Fluorescence and Absorbance AUC to the Study of Biological Macromolecules

机译：分析超速离心（AUC）：荧光和吸光度AUC应用于生物大分子研究的概述
- 作者：Garrett B. Edwards;Uma M. Muthurajan;Samuel Bowerman;Karolin Luger
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第133期
- analytical ultracentrifugation;
- FDS;
- fluorescence;
- macromolecular assembly;
- protein-DNA complex;
75.Rapid and Efficient Synthetic Assembly of Multiplex Luciferase Reporter Plasmids for the Simultaneous Monitoring of Up to Six Cellular Signaling Pathways

机译：多重荧光素酶报告组质粒的快速高效合成组装，用于同时监测多达六个细胞信号传导途径
- 作者：Alejro Sarrion-Perdigones;Yezabel Gonzalez;Koen J.T. Venken
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第131期
- assay;
- GoldenBraid;
- luciferase;
- multiplex;
- pathway reporter;
- synthetic DNA assembly;
- type IIS cloning;
76.Preparation of Mammalian Nascent RNA for Long Read Sequencing

机译：哺乳动物新生RNA的制备长读取测序
- 作者：Kirsten A. Reimer;Karla M. Neugebauer
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第133期
- chromatin;
- fractionation;
- long read sequencing;
- nascent RNA;
77.Optimized Transgene Delivery Using Third-Generation Lentiviruses

机译：使用第三代Lentiviruses优化的转基因递送
- 作者：Katherine P. Gill;Mark Denham
- 期刊名称：《Current Protocols in Molecular Biology》 | 2020年第133期
- high titer;
- lentiviral production;
- lipofection;
- ultracentrifugation;
- third-generation;
78.MHC-Peptide Tetramers to Visualize Antigen-Specific T Cells

机译：MHC-Peptide Tetramers to Visualize Antigen-Specific T Cells
- 作者：John D. Altman;Mark M. Davis
- 期刊名称：《Current Protocols in Molecular Biology》 | 2016年第115期
- MHC tetramers;
- T cells;
- flow cytometry;
79.Mitochondrial Ribosome (Mitoribosome) Profiling for Monitoring Mitochondrial Translation In Vivo

机译：Mitochondrial Ribosome (Mitoribosome) Profiling for Monitoring Mitochondrial Translation In Vivo
- 作者：Mary T. Couvillion;L. Stirling Churchman
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第119期
- mitochondria;
- ribosome profiling;
- ribosome immunoprecipitation;
- RNA-seq;
- northern blot;
- translation;
80.CRISPR/Cas9-Directed Gene Editing for the Generation of Loss-of-Function Mutants in High-Throughput Zebrafish Screens

机译：CRISPR/Cas9-Directed Gene Editing for the Generation of Loss-of-Function Mutants in High-Throughput Zebrafish Screens
- 作者：Sunita S. Shankaran;Timothy J. Dahlem;Brent W. BisgroveH. Joseph YostMartin Tristani-Firouzi
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第119期
- CRISPR/Cas9;
- F_0 screen;
- gene editing;
- loss-of-function;
- recessive mutant;
- zebrafish;
81.Transduction of Herpesvirus saimiri-Transformed T Cells with Exogenous Genes of Interest

机译：Transduction of Herpesvirus saimiri-Transformed T Cells with Exogenous Genes of Interest
- 作者：Rubén Martínez-Barricarte;Sarah Jill de Jong;Janet MarkleRoel de PausStephanie Boisson-DupuisJacinta BustamanteEsther van de VosseBernhard FleckensteinJean-Laurent Casanova
- 期刊名称：《Current Protocols in Molecular Biology》 | 2016年第115期
- herpesvirus saimiri-transformed T cells;
- HVS-transformed T cell;
- retrovirus;
- transduction;
- T cells;
82.Automated Computational Processing of 3-D MR Images of Mouse Brain for Phenotyping of Living Animals

机译：Automated Computational Processing of 3-D MR Images of Mouse Brain for Phenotyping of Living Animals
- 作者：Christopher S. Medina;Brett Manifold-Wheeler;Aaron GonzalesElaine L. Bearer
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第119期
83.NEBNext Direct: A Novel, Rapid, Hybridization-Based Approach for the Capture and Library Conversion of Genomic Regions of Interest

机译：NEBNext Direct: A Novel, Rapid, Hybridization-Based Approach for the Capture and Library Conversion of Genomic Regions of Interest
- 作者：Amy B. Emerman;Sarah K. Bowman;Andrew BarryNoa HenigKruti M. PatelAndrew F. GardnerCynthia L. Hendrickson
- 期刊名称：《Current Protocols in Molecular Biology》 | 2017年第119期

客服邮箱：kefu@zhangqiaokeyan.com

客服微信
服务号