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METHOD FOR PREPARING GENOME LIBRARY, AND GENOME LIBRARY PREPARED BY THE METHOD

机译:基因组文库的制备方法,以及用该方法制备的基因组文库

摘要

There is provided a first method for preparing a genome library in which PCR is carried out with the use of genomic DNA of a target biological organism itself or fragments thereof as a direct template and with the use of one kind of primer with a specified sequence or a random primer so as to effect genome amplification, thereby obtaining a genome library. There is further provided a second method for preparing a genome library in which genome of a target biological organism is pretreated and thereafter PCR is carried out with the use of one kind of primer with a specified sequence so as to effect genome amplification, thereby obtaining a genome library. In both of the methods, a genome library can be prepared conveniently from a small amount of sample.
机译:提供了一种制备基因组文库的第一种方法,其中使用靶生物本身的基因组DNA或其片段作为直接模板并使用一种具有指定序列或序列的引物进行PCR。随机引物以实现基因组扩增,从而获得基因组文库。还提供了第二种制备基因组文库的方法,其中,对目标生物的基因组进行预处理,然后使用一种具有指定序列的引物进行PCR,从而进行基因组扩增,从而获得目的基因。基因组文库。在这两种方法中,都可以方便地从少量样品中制备基因组文库。

著录项

  • 公开/公告号IN2006DN05078A

    专利类型

  • 公开/公告日2007-06-22

    原文格式PDF

  • 申请/专利权人

    申请/专利号IN5078/DELNP/2006

  • 申请日2006-09-04

  • 分类号C12N15/09;

  • 国家 IN

  • 入库时间 2022-08-21 20:57:56

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