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METHOD FOR PREPARING GENOME LIBRARY, AND GENOME LIBRARY PREPARED BY THE METHOD
METHOD FOR PREPARING GENOME LIBRARY, AND GENOME LIBRARY PREPARED BY THE METHOD
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机译:基因组文库的制备方法,以及用该方法制备的基因组文库
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摘要
There is provided a first method for preparing a genome library in which PCR is carried out with the use of genomic DNA of a target biological organism itself or fragments thereof as a direct template and with the use of one kind of primer with a specified sequence or a random primer so as to effect genome amplification, thereby obtaining a genome library. There is further provided a second method for preparing a genome library in which genome of a target biological organism is pretreated and thereafter PCR is carried out with the use of one kind of primer with a specified sequence so as to effect genome amplification, thereby obtaining a genome library. In both of the methods, a genome library can be prepared conveniently from a small amount of sample.
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