The process of preparing thermoactive alkaline lipase from lipolytic strain of Burkholderia multivorans, said process comprising the steps of: isolating lipolytic strain of B. multivorans from compost, culturing the said isolated B. multivorans in nutrient source, separating lipase by conventional methods, purifying the said separated lipase to electrophoretic grade, to obtain lipase that is stable and active in a wide range of temperature and pH, shows stability and activity in organic solvents, exhibits regioselectivity, enantioselectivity, wide substrate specificity and detergent compatibility
展开▼