DIRECT MOLECULAR CLONING OF A MODIFIED EUKARYOTIC CYTOPLASMIC DNA VIRUS GENOME

摘要

The invention provides a plasmid for producing amodified chordopoxvirus, wherein the plasmid comprises afirst master cloning site that includes unique sites of asecond master cloning site of the vaccinia virus vectordesignated vdTK, selected from the following group ofplasmids shown in Figure 4.3: pA0 comprising the sequence ofSEQ. ID. NO. 6, pA1 comprising the sequence of SEQ. ID. NO.7, and pA2 comprising the sequence of SEQ. ID. NO. 8.