SYSTEM FOR PRODUCING MULTIMERIC PEPTIDES AND PROTEINS AND USE THEREOF

摘要

The invention relates to a system based on a DNA construction comprising at least the following functionally bound sequences, namely: a nucleic acid sequence (A) containing the nucleotide sequence that codes for a product of interest, and a nucleic acid sequence (B) containing the nucleotide sequence that codes for a tetramerisation domain, such as the tetramerisation domain of protein p53, in which the 3' terminal of nucleic acid sequence A is bound to the 5' terminal of nucleic acid B. The system can be used to produce products of interest, for example recombinant multimeric peptides and proteins which can be used in vaccination, therapy, diagnosis, or industrial enzymes. Plants and insect larvae, transformed with the appropriate vectors such as to express the fused protein or peptide, are used as production systems. The expression of the 2L21 peptide from the canine parvovirus is illustrated in plants and the peptide binding to the dynein of protein p54 from African swine fever virus is illustrated in larvae, in both cases fused respectively to the p53 tetramerisation domain.