首页> 外国专利> DNA FRAGMENT USED IN THE FORM ATTACHED TO 5'-TERMINUS OF PRIMER FOR USE IN AMPLIFICATION REACTION OF NUCLEIC ACID, AND USE THEREOF

DNA FRAGMENT USED IN THE FORM ATTACHED TO 5'-TERMINUS OF PRIMER FOR USE IN AMPLIFICATION REACTION OF NUCLEIC ACID, AND USE THEREOF

机译:以与引物5'-末端附加的形式使用的DNA片段,用于核酸的扩增反应及其用途

摘要

A method is provided which enables quick, convenient, inexpensive, and high sensitivity confirmation of nucleic acid amplification after a nucleic acid amplification reaction. The DNA fragment in accordance with the present invention is a single-stranded DNA fragment containing a hairpin structure which in turn contains a bulge, wherein the DNA fragment is used as being attached to the 5' end of a primer used in nucleic acid amplification. The nucleic acid amplification confirmation method in accordance with the present invention quantifies a hairpin primer containing the DNA fragment at its 5' end by using bulge-binding fluorescent molecules after carrying out PCR or like nucleic acid amplification reaction using the hairpin primer. SNPs are detected quickly and conveniently at low cost and with high sensitivity by applying the nucleic acid amplification confirmation method, for example, to allele specific PCR.
机译:提供了一种在核酸扩增反应后能够快速,方便,廉价和高灵敏度确认核酸扩增的方法。根据本发明的DNA片段是包含发夹结构的单链DNA片段,该发夹结构又包含凸起,其中该DNA片段被用作与核酸扩增中所用引物的5'末端连接的形式。根据本发明的核酸扩增确认方法,在使用发夹引物进行PCR或类似的核酸扩增反应之后,通过使用凸起结合的荧光分子,对在其5'端包含DNA片段的发夹引物进行定量。通过将核酸扩增确认方法应用于等位基因特异性PCR,可以低成本,高灵敏度快速,方便地检测SNP。

著录项

  • 公开/公告号EP2058392A1

    专利类型

  • 公开/公告日2009-05-13

    原文格式PDF

  • 申请/专利权人 OSAKA UNIVERSITY;

    申请/专利号EP20070806129

  • 申请日2007-08-28

  • 分类号C12N15;C12N15/09;C12Q1/68;

  • 国家 EP

  • 入库时间 2022-08-21 19:15:37

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