Method that minimize cell hemolysis in human red blood cells exposed to high trehalose levels

摘要

The present invention relates to a process for Rose (trehalose) to high levels of the tree when freeze-dried cells in cell inde process is essential to a method for minimizing cell damage that occurs in this process. More particularly from outside the cell number of ways to impose a rose tree inside the cell as a secondary environment to create a rose tree, creating a high concentration rose to high levels in the tree. Existing methods rose tree buffer to the cell membrane (buffer) l, the crowd rose With this method hagieneun how this cell damage deep clinical use but just because it is imposed to secure the tree with minimal damage to the cells at high concentrations can be imposed. ; cell model has to select a human red blood cell experiment methods are red blood cells were cultured at 37 15 hours was observed for hemolysis phenomenon, according to various experimental conditions. Material and the concentration of the final cell protection protocols in this experiment to assist in the reduction of hemolysis when charged to a high concentration of a rose tree in cells 5 10 ^-4 M L-ascorbic acid, 5 10 ^-5 M -lipoic acid, 5 10 ^-5 M melatonin, 3 10 ^-4 M N-acetyl-L-cysteine , 10 ^-5 M -tocopherol and 5 10 ^{-5 was} M insulin. In this experiment, while imposing a rose tree to inside concentration of 60mM or more cells that require a combination of these materials with clinically using the results of hemolysis was observed for more than 5%. These results because the new ways you can help the survival of cells in special circumstances to impose a rose to a high concentration of a tree when freeze-dried cells to cells and applied for a patent for its contents.