RATE OF HEMOLYSIS OF FRESH AND STORED HUMAN RED BLOOD CELLS AND THE EFFECT OF PROGESTERONE.

摘要

The rate of hemolysis of human red blood cells has been studied by spectrophotometric measurements of the optical density of erythrocytes swelling and hemolyzing in water,sucrose solutions of varying concentration or sucrose solutions buffered with sodium phosphate buffer,pH 7.4. The rate of hemolysis of red cells,prepared from blood stored at 2C in acid-citrate-dextrose solution,increases with increased time of storage. The addition of physiological amounts of progesterone to red blood cells stored in the cold under sterile conditions greatly depresses the increase of the rate of hemolysis;red blood cells preserved for 40days with addition of progesterone manifest the same rate of hemolysis as that of red blood cells stored for 10days without progesterone. Fragility tests on red cells preserved with and without the addition of progesterone confirm these results;the addition of progesterone in the preservation of red blood cells helps to maintain the structural integrity of the cells. (Author)