FABRICATION METHOD OF AN IMMUNOSENSOR CHIP USING GOLD NANO-PARTICLES AND FUSION PROTEINS WITH A PORTION OF HIGH AFFINITY GOLD BINDING PROTEINS AND PROTEIN A OR G AND THEIR APPLICATIONS FOR DETECTION OF BIOMOLECULES

摘要

A sensor chip using the technique of surface Plasmon resonance (SPR) is provided to introduce a nano gold colloid without a probe, improve productivity and economic efficiency and increase the sensitivity of chip detection. A sensor chip using a surface plasmon resonance (SPR) comprises: a intermediate layer which is formed by introducing organic single molecule having a functional group of amine(-NH2) or thiol(-SH) functional group at the one side end and thiol(-SH) functional group at the other side end on substrate in which gold is applied or dotted; a gold colloid layer which is absorbed on the intermediated layer; a fusion protein in which a gold binding protein (GBP) and protein A or G is bonded to the gold colloid layer; and an antibody which is bonded to the fixed fusion protein.