Biocatalytic reduction of hydrophobic prochiral ketone compounds to chiral alcohols, comprises fixing prochiral ketones in adsorber, dissolving fixed material in aqueous medium and transporting into chamber in presence of microorganisms

摘要

Biocatalytic reduction of highly water-soluble hydrophobic prochiral ketone compounds (I) to chiral alcohols using microorganisms, comprises fixing the prochiral ketones in an adsorber, dissolving the fixed material in an aqueous medium and transporting into a reaction chamber in the presence of microorganisms capable of reducing (I) to chiral alcohols. Biocatalytic reduction of highly water-soluble hydrophobic prochiral ketone compounds of formula (R 1-C(=O)-R 2) (I) using microorganisms to chiral alcohols, comprises fixing the prochiral ketones in adsorber, dissolving the fixed material in an aqueous medium and transporting into a reaction chamber in the presence of microorganisms capable of reducing (I) to chiral alcohols. R1, R2 : alkyl, alkenyl, cycloalkyl, cycloalkenyl, aryl, aralkyl or cycloalkylalkyl (all optionally substituted by halogenated nitro and amino groups). An independent claim is included for a device for the biocatalytic reduction of (I) using microorganisms, comprising a bioreactor (01), a separately arranged adsorber column (02) and a cell retention filter (03), where the bioreactor, the adsorber column and the cell retention filter are connected with one another by a pipe (07) so that an aqueous reaction medium is supplied in the circulation.