METHOD FOR ASSAYING ACETYL-CoA CARBOXYLASE ACTIVITY, AND SCREENING METHOD USING THE FORMER METHOD

摘要

PROBLEM TO BE SOLVED: To provide a new method for easily assaying ACC (acetyl-CoA carboxylase) activity in high sensitivity without using a radioactive label, and a method for screening the ACC activity of a test substance used in the former method.;SOLUTION: The method for assaying ACC activity includes the following process: ADP formed by the enzymatic reaction of ACC carboxylase is made to generate hydrogen peroxide using pyruvate kinase and then the activity of the ACC is assayed to quantify the hydrogen peroxide. The method for screening the ACC activity using the former method is also provide.;COPYRIGHT: (C)2010,JPO&INPIT