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Wheat Plant comprising a DNA Construct conferred Enhanced tolerance to glyphosate

机译:包含DNA构建体的小麦植株对草甘膦的耐受性增强

摘要

In addition to providing Seeds, isolated DNA Molecules, Methods for Detecting The Inheritance of a DNA Molecule, a method to develop a tolerance to glyphosate in plants of Wheat, weed control method and a method for producing Seeds of Wheat.Claim 1: a Fertile glyphosate tolerant Wheat Plant produced by the method is characterized in that it comprises: (1) construct a DNA Construct comprising a First and a Second cassette expression, where the First Expression cassette includes United operat Selectively.(i) a Rice actin 1 Promoter intron; (ii) a Rice actin 1; (iii) to a chloroplast Transit Peptide that encodes a DNA Molecule; (iv) a DNA Molecule encoding a glyphosate tolerant EPSPS; and (V) a DNA Molecule of Paver Transcription; and such. While expression cassette includes United, operationally,(a) a CaMV 35S promoter Hsp70 intron; (b); (c) a DNA Molecule encoding a chloroplast Transit Peptide; (d) a DNA Molecule encoding a glyphosate tolerant EPSPS; and (e) a Molecule of DNA Transcription termination; and (2) transform a cell of Wheat The Construction of DNA; and (3) to regenerate the cell of Wheat in a Wheat Plant; and (4) TTreat these plants of wheat with an effective dose of glyphosate; and (5) select Fertile Wheat plants that are tolerant to glyphosate from the point of view of Vegetative and Reproductive.
机译:除了提供种子,分离的DNA分子,检测DNA分子的遗传的方法,发展对小麦植物中草甘膦的耐受性的方法,杂草控制方法和生产小麦种子的方法外,第1项:肥沃的土壤。通过该方法生产的耐草甘膦的小麦植物的特征在于,其包括:(1)构建包含第一和第二盒表达的DNA构建体,其中所述第一表达盒选择性地包括United操纵子。(i)水稻肌动蛋白1启动子内含子。 ; (ii)水稻肌动蛋白1; (iii)编码DNA分子的叶绿体转运肽; (iv)编码耐草甘膦的EPSPS的DNA分子; (V)摊铺机转录的DNA分子;等等。尽管表达盒在操作上包括United,(a)CaMV 35S启动子Hsp70内含子; (b); (c)编码叶绿体转运肽的DNA分子; (d)编码耐草甘膦EPSPS的DNA分子; (e)DNA转录终止分子; (2)转化小麦的细胞。 (3)在小麦植物中再生小麦的细胞; (4)用有效剂量的草甘膦处理这些小麦植株; (5)从营养和生殖角度出发,选择能耐受草甘膦的肥沃小麦植物。

著录项

  • 公开/公告号AR069294A2

    专利类型

  • 公开/公告日2010-01-13

    原文格式PDF

  • 申请/专利权人 MONSANTO TECHNOLOGY LLC;

    申请/专利号AR2008P104938

  • 申请日2008-11-12

  • 分类号A01H5/00;A01H5/10;C12N15/11;C12N15/29;

  • 国家 AR

  • 入库时间 2022-08-21 18:47:32

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