A METHOD FOR CO-PRODUCTION OF 1, 3-PROPANEDIOL, 2,3-BUTANEDIOL AND POLYHYDROXYPROPIONIC ACID BY FERMENTATION OF CONSTRUCTED GENETIC ENGINEERING BACTERIA

摘要

A method for co-production of 1,3-propanediol (PDO), 2,3-butanediol (BDO) and polyhydroxypropionic acid (PHP) via fermentation by genetic engineering bacteria comprises the following steps of: knocking out D type lactate dehydrogenase from wild PDO producing bacteria, introducing coenzyme A depending aldehyde dehydrogenase and polyhydroxy fatty acid synthase genes, constructing genetic engineering bacteria for producing PDO, BDO and PHP; fermenting aerobically and adjusting fermentation by adding mixture flow of glycerin and base solution; filtering fermentation broth by membrane, treating it by electrodialysis, concentrating, rectifying which separates PDO, BDO and PHP. The said genetic engineering bacteria can produce PDO, BDO and PHP at the same time, which increases utilization ratio of raw material, reduces cost, decreases generation of byproduct lactate, simplifies post extraction technique, lowers extraction cost, and increases generation of nicotinamide adenine dinucleotide 2 (NADH2) while PHP is introduced.