METHOD FOR EVALUATING PORK MEAT QUALITY USING A BIOMARKER FOR DETERMINING PORK MEAT QUALITY BY COMBINING PROTEOMICS AND METABOLOMICS

摘要

PURPOSE: A method for determining pork meat quality using a biomarker which is able to select pork of high quality is provided to reduce production cost by selecting and raising pork of high quality and to save time and cost for quality test.;CONSTITUTION: A biomarker which is able to select pork having high quality is creatine and creatine kinase. A method for developing the biomarker comprises: a step of extracting total protein from pork loin; a step of dissolving and extracting protein powder and centrifuging; a step of collecting supernatant and performing electrophoresis; a step of dyeing with silver and comparing protein expression; a step of isolating protein spot from the two-dimensional gel and performing hydrolysis; a step of analyzing proteome profiling from pork muscle between high pH and low pH groups; a step of analyzing serum sample of the high pH and low pH groups with 1H-NMR; and a step of confirming that the metabolite compositions are different between the groups.;COPYRIGHT KIPO 2010