METHOD FOR DETERMINATION OF GENETIC RELATIONSHIP OF COMMA BACILLI STRAINS BY SEQUENCING GENES FLANKING CLUSTER OF O-ANTIGEN BIOSYNTHESIS GENES

摘要

FIELD: medicine.;SUBSTANCE: method implies DNA recovery of an investigated strain, polymerase chain reaction (PCR) with oligonucleotide primers presented in the patent claim, and determination of a nucleotade sequences of amplified rjg, gmhD and orf2 gene fragments and their comparison to similar genes of comma bacilli stains of the others serogroups. The genetic relationship of comma bacilli stains of various origins is determined by a number of individual nucleotide substitutions in rjg, gmhD and orf2 genes flanking a cluster of O-antigen biosynthesis, in comparison similar genes of comma bacilli stains of the others serogroups presented in GenBank database.;EFFECT: use of the invention allows for rapid, effective and reliable determination of relationship of comma bacilli stains of various origins with the other comma bacilli, including with virulent and epidemically significant vibrios of O1 and O139 serogroups.;2 tbl, 8 ex