RECOMBINANT PLASMID DNA pL-ASP-08 AND Escherichia coli XL1-blue/pL-ASP-08 BACTERIA STRAIN - L-ASPARAGINASE PRODUCER

摘要

FIELD: chemistry; biochemistry.;SUBSTANCE: invention relates to biotechnology, particularly to genetic engineering and pertains to a novel plasmid pL-ASP-08 and a novel Escherichia coli XL 1-blue/pL-ASP-08 bacteria strain containing the said plasmid. The recombinant plasmid DNA pL-ASP-08 has molecular weight of 2.9 MDa (4471 base pairs) and is designed for L-asparaginase synthesis.;EFFECT: invention enables to obtain highly pure genetically engineered L-asparaginase for preparing medicinal agents used in treating lymphoblastic leucosis, lympho- and reticulosarcoma in combined chemotherapy.;2 cl, 1 dwg, 2 ex