RECOMBINANT PLASMID DNA pHIASP03, ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHIAsp03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIAsp03 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart

摘要

FIELD: biotechnology.;SUBSTANCE: invention is a recombinant plasmid DNA pHIAsp03, directing the synthesis of human hybrid protein with human proinsulin Aspart containing DNA encoding the hybrid protein with human proinsulin Aspart with size of 134 amino-acid residue with the amino acid sequence shown in Figure 2, comprising the amino acid sequence of the leader peptide in the form of N-terminal fragment of human gamma-interferon, connected to proinsulin Aspart with the peptide linker. On the basis of the recombinant plasmid pHIAsp03 the strain Escherichia coli JM109/pHIAsp03 was obtained - producer of the hybrid protein with proinsulin Aspart, registered in the federal state unitary enterprise State Research Institute of Genetics RNCIM under the number B-11411.;EFFECT: use of the claimed inventions enables to simplify the technology of isolation of insulin Aspart and to increase its yield.;4 cl, 2 dwg, 2 ex