RECOMBINANT PLASMID DNA pHIG05, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA PHIG05, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIG05-PRODUCENT OF HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine

摘要

FIELD: biotechnologies.;SUBSTANCE: invention represents a recombinant plasmid DNA pHIG05, guiding synthesis of hybrid human protein with human proinsulin Glargine, containing DNA that codes hybrid protein with human proinsulin Glargine with size of 134 a.o. with amino acid sequence shown in fig.2, containing amino acid sequences of a leader peptide, in the form of an N-end fragment of human gamma-interferon connected to human proinsulin Glargine by peptide linker. On the basis of the recombinant plasmid pHIG05 a strain Escherichia coli JM109/pHIG05 is produced - producer of hybrid protein with proinsulin Glargine, registered in the Federal State Unitary Enterprise GosNIIgenetikie VKPM under No.V-11408.;EFFECT: usage of proposed inventions makes it possible to simplify technology of Glargine insulin extraction and to increase its yield.;4 cl, 2 dwg, 2 ex