mutated single stranded dna binding protein that is highly thermophil and its use in a isothermalen nucleic acid amplifizierungsmethode

摘要

PROBLEM TO BE SOLVED: To establish a technique by which nonspecific amplification is controlled in the nucleic acid amplification in an isothermal amplification reaction to improve amplification accuracy.;SOLUTION: The modified single-stranded DNA-binding protein derived from an extremely thermophilic bacterium has an amino acid sequence exhibiting a function contributing to the improvement of amplification efficiency of a template nucleic acid in an isothermal amplification system using a chain-substituting polymerase. The protein has a modified site at which at least one of modification of deletion, substitution, addition and insertion of one or more amino acids is caused.;COPYRIGHT: (C)2007,JPO&INPIT