Interaction of the Escherichia coli single-stranded DNA-binding protein with nucleic acids and its comparison with protamine.

摘要

Single-stranded DNA binding proteins or SSBs are nonspecific, single-stranded DNA binding proteins. They play a role in DNA replication, recombination, and repair. They are also referred to as unwinding, melting or helix-destabilizing proteins. SSBs are able to protect single-stranded DNA from nuclease digestion. Protamines are a class of low-molecular-weight proteins that protect the chromosomal DNA in the sperm cells of eukaryotic organisms. Protamines package the sperm DNA into a highly compact state and render it genetically inactive.;The binding properties of Eco SSB to single-stranded DNA have been integrated, correlated, and evaluated. The influence of different factors such as salt concentration, effect of ions, and temperature on the interaction of Eco SSB to DNA have been discussed. Finally, a comparison of the DNA-SSB binding with DNA-protamine binding has been presented.