The method and apparatus polymorphism detection reaction vessel, gene

摘要

PROBLEM TO BE SOLVED: To provide a reaction vessel useful as a reagent kit suitable for automation of various measurements.;SOLUTION: In a preferable form, a resin plate 40 is covered with an elastic film 42, a sample injection part 44 equipped with a cap 18 of a diaphragm structure, a PCR reaction reagent storage part 46, a typing reagent storage part 48 and a mixing part 50 are formed on the elastic film. An organism sample is injected into the sample injection part 44, a stopper 58 is removed, a proper part of a flow channel is closed, the sample injection part 44 and the PCR reaction reagent storage part 46 are alternately pushed, the sample is mixed with the PCR reaction reagent and retained in a PCR reaction part 54 to carry out a PCR reaction by a fixed temperature cycle. Then, a proper part of the flow channel is closed, the typing reagent storage part 48 and the mixing part 50 are alternately pushed, the reaction liquid is mixed with the typing reagent, the reaction solution is sent to a probe arrangement part 30 and a fluorescence is detected by a corresponding probe to detect SNP.;COPYRIGHT: (C)2006,JPO&NCIPI