The present invention is related to a method for inducing in vitro the differentiation of stem cells or somatic cells towards germinal cells, comprising the following steps:cultivating said stem cells or somatic cells in a medium allowing their differentiation, andcollecting the germinal cells from the culture medium,wherein said cells are cells transformed with an exogenous genetic construction comprising at least a Vasa gene.;Germinal cells such as obtained and chimeric animals are also an object of this invention.
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