首页> 中文期刊> 《中国神经再生研究:英文版》 >Reprogramming of mouse neural stem cells to induced pluripotent stem cells using Oct4 combined with microRNA

Reprogramming of mouse neural stem cells to induced pluripotent stem cells using Oct4 combined with microRNA

         

摘要

microRNA is important for maintaining characteristics of embryonic stem cells,and microRNA302a(MiR-302a) has been shown to exert important effects on cell reprogramming.Therefore,the present study used miR294 and miR302a,in combination with Oct4,to induce mouse neural stem cells(NSCs) into induced pluripotent stem(iPS) cells.Following identification of iPS cells,the effects of microRNA on cell reprogramming were analyzed.Results suggested that reprogramming efficiency with Oct4 + miR-294 + miR-302a was 7-fold greater than Oct4 alone(0.1% vs.0.014%).The iPS cells were undifferentiated and positive for alkaline phosphatase,SSEA-1,and Oct4.These findings demonstrated that microRNAs play an important role in cell reprogramming and provide a safe and efficient induction system for cellular reprogramming.

著录项

获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号