首页> 外国专利> METHOD FOR RECOVERING NUCLEIC ACID FROM STOOL SAMPLE, NUCLEIC ACID ANALYSIS METHOD AND STOOL SAMPLE PROCESSING APPARATUS

METHOD FOR RECOVERING NUCLEIC ACID FROM STOOL SAMPLE, NUCLEIC ACID ANALYSIS METHOD AND STOOL SAMPLE PROCESSING APPARATUS

机译:从商店样品中回收核酸的方法,核酸分析方法和商店样品处理装置

摘要

The present invention relates to the a nucleic acid analysis method that uses nucleic acid recovered according to the nucleic acid recovery method, and a stool sample processing apparatus used in that method, wherein the method has steps of: (A) eluting nucleic acid amplification reaction inhibitory substances by preparing a stool sample by adding a collected stool to a solution for removing nucleic acid amplification reaction inhibitory substances that has a water-soluble organic solvent as an active ingredient thereof, and storing the stool sample for a predetermined time, (B) recovering a stool-derived solid fraction by removing the solution for removing nucleic acid amplification reaction inhibitory substances from the stool sample after step (A), and (C) recovering nucleic acid from the stool-derived solid fraction recovered in step (B).
机译:本发明涉及使用根据该核酸回收方法回收的核酸的核酸分析方法,以及在该方法中使用的粪便样品处理设备,其中该方法具有以下步骤:(A)洗脱核酸扩增反应。通过将收集的粪便添加到用于去除核酸扩增反应抑制物质的溶液中以制备粪便样本的抑制物质,所述核酸扩增反应抑制物质具有水溶性有机溶剂作为其活性成分,并将粪便样本储存预定时间,(B)通过从步骤(A)之后的粪便样品中除去用于去除核酸扩增反应抑制物质的溶液,回收粪便来源的固体成分,以及(C)从步骤(B)中回收的粪便来源的固体成分中回收核酸。

著录项

  • 公开/公告号US2011183332A1

    专利类型

  • 公开/公告日2011-07-28

    原文格式PDF

  • 申请/专利权人 YASUO TANIGAMI;TOMONORI NAGAOKA;

    申请/专利号US201113012442

  • 发明设计人 YASUO TANIGAMI;TOMONORI NAGAOKA;

    申请日2011-01-24

  • 分类号C12Q1/68;C07H1/06;C07H21/00;G01N33/48;

  • 国家 US

  • 入库时间 2022-08-21 18:13:45

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