'Method for the relative quantification of at least one target nucleic acid in a sample, method for absolute quantification of at least one target nucleic acid in a sample, method for determining the value of at least one target nucleic acid in a sample, means machine readable equipment and nucleic acid sample analysis equipment '

摘要

The invention relates to a method for quantification of amplified nucleic acids comprising the steps of: calculation of a measure of randomness M of the cycle-to-cycle amplification efficiency Ê(C) for target and comparative nucleic acids,identification of the cycle numbers CM where M is minimal for target and comparative nucleic acids, calculation of the characteristic cycle numbers Cc from the values of CM-.