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METHOD FOR THE CONSTRUCTION OF RANDOMIZED GENE SEQUENCE LIBRARIES IN CELLS

机译：细胞中随机基因序列文库的构建方法

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摘要

An in vivo method for the construction of randomized gene libraries and/or domain replacement in gene libraries by homologous recombination using a Kluyveromyces lactis killer toxin, in particular the γ-subunit of the K. lactis killer toxin, as negative selection marker is described. The use of the γ-subunit of K. lactis as negative selectable marker increases the percentage of randomized clones.

机译：一种体内方法，可使用乳酸克鲁维酵母（Klyveromyces lactis）杀伤性毒素，特别是 K的γ亚基，通过同源重组在基因库中构建随机基因库和/或在基因库中进行域替换。描述了乳酸菌杀手毒素作为阴性选择标记。使用的γ-亚基。乳酸作为阴性选择标记会增加随机克隆的百分比。 展开▼

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公开/公告号US2011020912A1

专利类型

公开/公告日2011-01-27

原文格式PDF

申请/专利权人 CLAUDIA SCHÄRER-BRODBECK;
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申请/专利号US20100901785

发明设计人 CLAUDIA SCHÄRER-BRODBECK;
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申请日2010-10-11

分类号C12N1/19;C12N15/63;

国家 US

入库时间 2022-08-21 18:12:57

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