Selective amplification method of mitochondrial DNA fragment in horses

摘要

the solution concerns the way the selective amplification fragment mtdna in horses, which is of significance in the detection pru00edbuzensku00fdch relation and evolucnu00edch studies.the selective principle is oligonukleotidu sequence, which are used as the new primers for the polymerase with a chain reaction on the composition of the forward primer 5 '- tctagctccaccatcaac - 3',the reverse primer 5 '- attgggacacgtagttgg - 3.