Selective amplification method of mitochondrial DNA fragment in horses

摘要

The present invention relates to a method for selectively amplifying a fragment of mtDNA in horses, which is important in the identification of relationships and evolutionary studies. The selective principle consists in the sequence of oligonucleotides that are used as new primers for a polymerase chain reaction on the composition of forward primer 5'-3'-tctagctccaccatcaac, Reverse primer 5'-3'-attgggacacgtagttgg.