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PROCESS FOR OBTAINING NUCLEIC ACIDS BY LYSE MICROBIAL SAMPLES
PROCESS FOR OBTAINING NUCLEIC ACIDS BY LYSE MICROBIAL SAMPLES
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机译:通过裂解微生物样品获得核酸的方法
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摘要
Methods and compositions for releasing the nucleic acids from a variety of different types of microorganisms are provided. The method relies on a simplified lysis procedure that can be applied to many types of bacteria and fungal cells and is readily automated for high throughput screening methods. The method utilizes a high concentration of a chelating agent and a mixture of lysing enzymes to accomplish the disruption of microbial cell walls and allow the release of the nucleic acid.
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