首页> 外国专利> Methods to regulate the expression of genes in transgenic plant cells transformed from a stable, plants transformed, molecules of nucleic acids isolated polipeptu00ecdeos maf1 and nmp1,Fusion genes, plant cells processed microbial cells processed, methods of modification of the degree of expression of proteu00ecnas connecting to mfp1 and maf1 in host cells.Methods of obtaining molecules of nucleic acids coding for the proteu00ecnas connecting to mfp1 and maf1 plants and methods for the assessment of at least one compound quu00ecmico as to their capacityAde to inhibit the activity of proteu00ecnas connecting to mfp1 and maf1 plants encoded by nucleic acid molecules.

Methods to regulate the expression of genes in transgenic plant cells transformed from a stable, plants transformed, molecules of nucleic acids isolated polipeptu00ecdeos maf1 and nmp1,Fusion genes, plant cells processed microbial cells processed, methods of modification of the degree of expression of proteu00ecnas connecting to mfp1 and maf1 in host cells.Methods of obtaining molecules of nucleic acids coding for the proteu00ecnas connecting to mfp1 and maf1 plants and methods for the assessment of at least one compound quu00ecmico as to their capacityAde to inhibit the activity of proteu00ecnas connecting to mfp1 and maf1 plants encoded by nucleic acid molecules.

机译:调节从稳定的,转化的植物的分子中分离出的核酸分子polipept maf1和nmp1的基因在转基因植物细胞中表达的方法,融合基因,植物细胞加工的微生物细胞,修饰基因表达度的方法连接到宿主细胞中mfp1和maf1的蛋白质。获得编码连接到mfp1和maf1植物的蛋白质的核酸分子的方法,以及评估至少一种化合物抑菌能力的方法连接到由核酸分子编码的mfp1和maf1植物的蛋白质的活性。

摘要

"Methods to regulate the expression of genes in transgenic plant cells transformed from a stable, plants transformed, molecules of nucleic acids isolated polipeptu00ecdeos maf1 and nmp1,Fusion genes, plant cells processed microbial cells processed, methods of modification of the degree of expression of proteu00ecnas connecting to mfp1 and maf1 in host cells.Methods of obtaining molecules of nucleic acids coding for the proteu00ecnas connecting to mfp1 and maf1 plants and methods for the assessment of at least one compound quu00ecmico as to their capacityAde to inhibit the activity of proteu00ecnas connecting to mfp1 and maf1 plants encoded by nucleic acid molecules.The present invention relates to nucleic acid molecules encoding plant proteins that interact with nuclear matrix proteins and function as transcription activators.If you use mfp1 and screening of yeast two hybrid, maf1 and nmp1 were isolated and placed in sequ00b3u00eancia and determined as new.If you use maf1 for a second yeast two hybrid screening, four additional new proteins have been isolated, placed in sequ00b3u00eancia and identified as flip1, flip2, flip3 and flip4.The proteins of this invention may be used to increase the level of expression of genes in plants and other eukaryotic organisms.
机译:调节转基因植物细胞中基因表达的方法,该基因由稳定的,转化的植物,分离的核酸分子,脂蛋白maf1和nmp1,融合基因,植物细胞处理的微生物细胞,修饰度的方法连接到mfp1和maf1的蛋白在宿主细胞中的表达。获得编码连接到mfp1和maf1的植物的蛋白的核酸分子的方法以及评估至少一种化合物qum的能力的方法抑制连接到由核酸分子编码的mfp1和maf1植物的蛋白质的活性。本发明涉及编码与核基质蛋白相互作用并起转录激活剂作用的植物蛋白的核酸分子。分离出两个酵母杂交体maf1和nmp1并将其置于seq u00b3 u00eancia中并确定为新的。在第二次酵母的两次杂交筛选中,已分离出另外四个新蛋白质,置于seq u00b3 u00eancia中,并鉴定为flip1,flip2,flip3和flip4。本发明的蛋白质可用于提高植物中基因的表达水平。和其他真核生物。

著录项

  • 公开/公告号BR9915307A

    专利类型

  • 公开/公告日2001-10-23

    原文格式PDF

  • 申请/专利权人 E.I. DU PONT DE NEMOURS AND COMPANY;

    申请/专利号BR19999915307

  • 发明设计人 FRANK GINDULLIS;IRIS MEIER;

    申请日1999-11-04

  • 分类号C12N15/82;C07K14/415;C12N15/29;C12Q1/02;C12Q1/68;G01N33/68;

  • 国家 BR

  • 入库时间 2022-08-22 01:25:57

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