Methods and kits of releasing cells are provided. The method comprises the steps of providing cultured cells on a cell culture support comprising a multi layer polyelectrolyte coating immobilized on a substrate, and releasing the cultured cells from the cell culture support by a releasing solution comprising DMSO. The kit comprises a cell culture support and a releasing solution. The cell culture support comprises a substrate and a multi layer polyelectrolyte coating immobilized on the substrate and the releasing solution comprises DMSO.
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