A Nanopore Biomimetic Device Quantitatively Detects Early Stage Cancer Cells; a Contour Map of Multiple Variable Correlation Method Assesses the Heat Released by Cancer Cells

摘要

We report a portable early stage low abundance cancer cells detection device that has the capability to detect the breast cancer MDA-MB-231 line in a single cell concentration quantitatively using the unique land marker ratio of "action potential" vs. "resting potential" (RAPRP) values that distinguish from the normal breast cells under antibody-free and labeling-free conditions. The device is based on a nanopore biomimetic cross linked polymer membrane fabricated on gold chips. This real time potential was recorded using a double step chronopotentiometry (DSCPO) under a fixed current. The sensitivity of the device to detect cancer cells is 530 mV/cell (ml/~(-1)) over 1-50 cell/mL range after 24 hrs incubation. Factors of cancer cell concentration and current affect on the DSCPO profiles are reported. The heat released by the cancer cells is an order of magnitude higher in calories compared with a normal breast cell assessed by a Contour Map of Multiple Variable Correlation method (CMMVC).