SYNTHESIS OF ETHYL (3R, 5S)-DIHYDROXY-6-BENZYLOXY HEXANOATE

摘要

A process for preparing ethyl (3R, 5S)-dihydroxy-6-benzyloxy hexanoate via stereoselective microbial reduction is provided, which includes that ethyl 3,5-diketo-6-benzyloxy hexanoate is used as a substrate and a diketoreductase is used as biocatalyst. Specifically, the process includes these following steps: (1) the substrate, diketoreductase and cofactor regeneration system mediated by formate dehydrogenase are added to the reaction liquid and conducted to shake and react for at least one hour, and the reaction liquid is the mixture of organic solvent and buffer solution; (2) the product of step (1) is separated and purified to obtain the single enantiomer of (3R, 5S)-dihydroxy compound.