Optimization of ethyl hexanoate synthesis in organic solvent by immobilized lipase from mucor miehei

摘要

A three-lever and three-variable experiment design of RSM (response surface methodology) was involved in evaluating the effects of synthetic variables, such as substrate molar ratio (1:1-1:2 hexanoic acid:ethanol), time (1.5-9.5hr), and temperature (10-50 deg C) at 0.40M substrate concentration, 0.2 percent added water, and 0.2g lipase in 15ml heptane. The three variables and their interaction were the important factors effecting the conversion of the synthetic reaction. Based on established model and contour plots, optimum conditions were: substrate molar ratio(hexanoic acid:ethanol)1:1.15, time 7.1hour, and temperature 28.2 deg C. The predicted value was identified to be able to evaluate the percent molar conversion of the synthetical reaction.