METHOD OF DETERMINING RELATIVE CONTENT OF SULPHATED FORMS OF GLYCOSIDES IN HOLOTHURIAN PREPARATIONS

摘要

FIELD: chemistry.;SUBSTANCE: invention can be used to determine relative content of sulphated forms of glycosides in holothurian preparations. Determination is done without separating the mixture of glycosides from HSQC NMR experiment data and with value of the τ₂ parameter (time delay between 180° and 90° pulses), determined from the relationship 2τ₂=1/(3¹J_ch), where ¹J_ch is the spin-spin interaction constant of carbon and its proton. Volumetric intensities of corresponding protons are then measured for substituted cross-peak carbons, and percentage content of components of the mixture is calculated from the obtained values by constructing and solving a system of linear equations.;EFFECT: possibility of analysing a mixture of glycosides without their pre-separtion on mono-, di- and tri-sulphated glycosides.;2 dwg