METHOD OF EVALUATING ESTROGEN DEPENDENCE OF HIGH-DIFFERENTIATED THYROID CANCER

摘要

FIELD: medicine.;SUBSTANCE: tissue samples are incubated at 37°C for 4 hours with estradiol in the concentration 2.21x10^-6 M, precipitated with dihitonine for one night at 4°C, and a homogenate of a check sample and a sample cultivated with estrogen is analysed for thyroid peroxidase (TPO) activity by spectrophotometry. If the enzymatic activity after co-incubation with estradiol is increased in the tumour tissue sample by at least 50 % in comparison with the reference, such growth is considered to be sensitive to estrogen action. Such conclusion is made regardless of the content of cytoplasmic receptors of steroid hormones in the analysed tissue.;EFFECT: use of the method allows evaluating estrogen-dependence of thyroid cancer in prescribing a conservative therapy of the disease.;2 tbl, 2 ex