SELECTIVE DIFFERENTIAL-DIAGNOSTIC MEDIUM FOR RECOVERING ANTHRAX AGENT AND RELATED Bacillus cereus BACILLI

摘要

FIELD: medicine.;SUBSTANCE: invention refers to preparation and use of a microbiological selective differential-diagnostic medium for recovering an anthrax agent Bacillus anthracis and related Bacillus cereus bacilli. The medium contains Hottinger's agar as a nutrient base (1), ceftazidime (18-22 mg) and amphotericin B (8-12 mg) for associated microflora growth inhibition and para-Nitrophenylphosphate (p-NPP) disodium salt which is a substratum for alkaline phosphatase enzymosis as a differential-diagnostic additive in the amount 490-510 mg. Differentiation is shown by the condition of the nutrient medium: colour of agar surrounding the Bac. anthracis colonies does not change, whereas the thickness of agar surrounding related colonies gets sharp yellow colour.;EFFECT: invention allows higher selectivity of Bacillus cereus growth and differentiating properties of the medium, eliminated irritant action of ammonia vapour on the researching personnel, facilitating result record.;1 tbl, 3 ex