EXPRESSION PLASMID DNA p6E-tTF CODING EXTRACELLULAR AND TRANSMEMBRANE DOMAINS OF HUMAN TISSUE FACTOR AND E.coli BL21 DE3/p6E-tTF STRAIN - PRODUCER OF RECOMBINANT HUMAN TISSUE FACTOR

摘要

FIELD: medicine.;SUBSTANCE: polypeptide contains extracellular and transmembrane domains of a human tissue factor. A DNA fragment coding this polypeptide, and a plasmid pET28a(+) are used to produce a genetic make-up enabling biosynthesis of the recombinant human tissue factor. Also, the E coli BL21 [DE3]/p6E-tTF strain that is a producer of the recombinant human tissue factor is offered.;EFFECT: high-yield recombinant protein and simplified recovery and purification procedures.;2 cl, 4 dwg, 3 ex