System and method for absolute quantification of proteins using the LC / MS

摘要

Absolute fixed quantity of the protein in the sample is brought N which is surveyed vis-a-vis the peptide of the protein of specification harmony or average of the highest ionization strength by comparing with calibration standard. To be something of shape of the table which is formed by the preceding protein peptide analysis which is done making use of the specified protein of one or more it is possible this calibration standard. This comparison is used although the absolute quantity to which the protein corresponds on the basis of harmony or the average where ionization strength is surveyed is calculated. Applying simple transfer coefficient to calibration standard value, it is possible to calculate the absolute quantity of the protein in the sample.