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SYSTEM AND METHOD FOR ABSOLUTE QUANTITATION OF PROTEINS USING LC/MS
SYSTEM AND METHOD FOR ABSOLUTE QUANTITATION OF PROTEINS USING LC/MS
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机译:使用LC / MS绝对定量蛋白质的系统和方法
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摘要
Absolute quantitation of protein in a saηiple is provided by comparing a sum or average of the N highest ionization intensities observed for peptides of a particular protein along with a calibration standard. The calibration standard can be in the form of a table generated by prior protein peptide analysis performed using one or more pre-determined proteins. The comparison is used to determine a corresponding absolute quantity of protein based on the observed sum or average of ionization intensities. A simple conversion factor can be applied to the calibration standard value to determine the absolute quantity of protein in the sample.
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