New carbonyl reductase, (a) below that gene

摘要

The present invention provides a novel polypeptide that produces (s) - n-benzyl-3-pyrrolidinol, a DNA encoding the same, and a method of using the same. Polypeptides having the physicochemical properties of the following (1) to (5): (1) action of NADPH as coenzyme; (1) - n-benzyl-3-pyrrolidinone with asymmetric reduction (s) - (n-benzyl-3-pyrrolidinol), (2) action pH: 4.5 to 5.5, 3 (3) the optimum temperature: 40 to 45 u2103, (4) molecular weight: About 29000 in gel filtration analysis, about 35000 in SDS polyacrylamide electrophoresis analysis, and (5) inhibitor: divalent copper ion. In addition, a polypeptide consisting of an amino acid sequence represented by sequence number 1 of the array table, or one or more amino acids in an amino acid sequence indicated by sequence number 1 of the array table, substituted, deleted, and / or added amino It is a polypeptide consisting of an acid sequence and an enzymatic activity to produce (s) - n-benzyl-3-pyrrolidinol by asymmetric reduction of n-benzyl-3-pyrrolidinone.