Genetically engineered pyrrolo quinoline quinone-dependent glucose dehydrogenase comprises an amino acid insertion

摘要

The present invention, soluble pyrroloquinoline quinone comprising an amino acid insertion between the # 429 and # 428 that corresponds to the known amino acid sequence of Acinetobacter calcoaceticus of (Acinetobactor calcoaceticus) derived from (PQQ)-dependent glucose dehydrogenase (s-GDH particularly protein and s-GDH variants such with a substrate improved specificity genes encoding variants with improved), variant sGDH such, for glucose, glucose in the sample, the concentration of glucose in particular on the application of different s-GDH variants of these to determine the.