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BETA-GLUCAN-DEFICIENT GENE IN BARLEY, SYNTHETIC GENE, AND USE OF SAME
BETA-GLUCAN-DEFICIENT GENE IN BARLEY, SYNTHETIC GENE, AND USE OF SAME
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机译:大麦中的β-葡聚糖缺失基因,合成基因及其用途
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摘要
A Genomic DNA and a cDNA of a β-glucan-deficient gene in a barley and of a β-glucan synthesis gene in a barley, a barley having the genomic DNA of a β-glucan-deficient gene and a method for breeding the same, a method for producing an alcohol or a fermented food using barley kernels decreasing or deficient in β-glucan, and an animal feed composition are provided. A Genomic DNA of a β-glucan-deficient gene consisting of a base sequence having 90% or more homology to the base sequence of SEQ ID NO: 1 and producing a protein lacking a β-glucan synthesis activity when transcribed and translated, and a breeding method comprising a step of selecting a barley by determining the barley as having a β-glucan-deficient gene when the base corresponding to position 4,275, or corresponding to position 2,385, of the gene consisting of the base sequence of SEQ ID NO: 4 located near the centromere of chromosome 7H of the barley is mutated from G to A.
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机译:大麦中的β-葡聚糖缺陷基因的基因组DNA和cDNA以及大麦的β-葡聚糖合成基因的基因,具有β-葡聚糖缺陷的基因的基因组DNA的大麦及其繁殖方法提供了使用减少或缺乏β-葡聚糖的大麦仁生产醇或发酵食品的方法,以及动物饲料组合物。 β-葡聚糖缺陷基因的基因组DNA,其由与SEQ ID NO:1的碱基序列具有90%或更高的同源性的碱基序列组成,并产生在转录和翻译时缺乏β-葡聚糖合成活性的蛋白质。育种方法包括以下步骤:通过选择大麦为具有β-葡聚糖缺陷基因的大麦,所述大麦具有与SEQ ID NO:4的碱基序列相对应的基因的4,275位或2,385位位于大麦7H染色体着丝粒附近的基因从G突变为A.
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