PCR-SEQUENCING METHOD BASED ON TECHNOLOGY OF DNA MOLECULAR INDEX AND STRATEGY OF DNA-BREAKING INCOMPLETELY

摘要

The present invention provides a sequencing method for nucleic acid, which method comprises: amplifying the target nucleic acid using indexed primers, breaking the amplicons incompletely and purifying and recovering mixed DNAs, sequencing the mixed DNAs by a next-generation sequencing method to obtain the sequences of the broken DNAs, and assembling the sequences of the broken DNAs into a complete sequence for the target nucleic acid. The present invention further provides primer indexes for the sequencing method.