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PCR sequencing method based on DNA molecular tag technology and DNA incomplete fragmentation technology and HLA genotyping method using the same
PCR sequencing method based on DNA molecular tag technology and DNA incomplete fragmentation technology and HLA genotyping method using the same
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机译:基于DNA分子标签技术和DNA不完全断裂技术的PCR测序方法以及使用其的HLA基因分型方法
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摘要
The invention provides a PCR sequencing method, wherein the combination of primer indexes, DNA incomplete shearing strategy and the second generation sequencing technique (Paired-End sequencing technique) can make the length of PCR products that can be sequenced by a sequencer longer than the maximum sequencing length of the sequencer whilst making full use of the characteristics of the second generation sequencing technique such as high throughput and low cost, thereby greatly broadening its applicable scope. In addition, the present invention also provides primer indexes for the PCR sequencing method and the use of the method in genotyping, particularly in HLA analysis, and also provides the PCR primers used, particularly the PCR primers for HLA-A, B, HLA-C and HLA-DQB1 gene.
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