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METHOD FOR DETECTING NUCLEIC ACIDS USING A NUCLEIC ACID ENZYME/MOLECULAR BEACON

机译:用核酸酶/分子信标检测核酸的方法

摘要

The present invention relates to a method for detecting nucleic acids using a nucleic acid enzyme/molecular beacon composite and a nucleic acid signal amplification method, and more particularly, to a method for detecting target nucleic acids using a composite of a nucleic acid enzyme having peroxidase activity and a molecular beacon having a hairpin structure, and the reaction of blocker nucleic acids and nucleic acid polymerase. The method for detecting nucleic acids according to the present invention enables target nucleic acids to be repeatedly used in the process of signal generation through color reaction using the strand displacement activity of nucleic acid polymerase, thus detecting target nucleic acids with high sensitivity, and therefore the method of the present invention may be applied to the detection of proteins and small molecules, as well as to the detection of nucleic acids.
机译:本发明涉及使用核酸酶/分子信标复合物的核酸检测方法和核酸信号扩增方法,更具体地,涉及使用具有过氧化物酶的核酸酶的复合物检测靶核酸的方法。活性和具有发夹结构的分子信标,以及阻断剂核酸和核酸聚合酶的反应。根据本发明的核酸检测方法,能够利用核酸聚合酶的链置换活性通过显色反应在信号产生过程中重复使用靶核酸,从而以高灵敏度检测靶核酸,因此可以本发明的方法可以应用于蛋白质和小分子的检测以及核酸的检测。

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