FUNCTIONAL CONSTRUCTION OF SECB-MEDIATED POST-TRANSLATIONAL TARGETING PATHWAY AND USE THEREOF

摘要

Provided is a functional construction of SecB-mediated post-translational targeting pathway and the use thereof. In particular, provided are a method for constructing a chimeric SecA protein; coexpressing the chimeric SecA protein and SecB protein in a bacterium, thus increasing the protein secretion efficiency of a secB gene deleted bacterium; a method for constructing a SecB-mediated post-translational targeting pathway in the secB gene deleted bacterium; and a method for improving the protein secretion ability of the secB gene deleted bacterium. Furthermore, also provided are a related amino acid sequence, nucleotide sequence, expression vector, and a system containing the amino acid sequence, nucleotide sequence and/or expression vector.