DJ-1 KNOCKOUT CELL LINE FOR OBTAINING A PATHOGENETIC MARKER USED FOR PARKINSON'S DISEASE DIAGNOSIS

摘要

PURPOSE: A DJ-1 knockout cell line which is able to detect pathogenetic marker of Parkinson's disease is provided to treat, prevent and relieve Parkinson's disease.;CONSTITUTION: A method for preparing DJ-1 knockout dopaminergic neural cell line KCTC 11472BP comprises: a step of crossbreeding TH-TagA58 transgenic mouse and DJ-1 gene targeting mouse to prepare a dual gene targeting mouse; a step of collecting and establishing DJ-1 gene knockout cell line from the substantia nigra of the dual gene targeting mouse; and a step of culturing the cell line at pH7.0 and 33°C.;COPYRIGHT KIPO 2012