Nucleic acid sequencing-based methods of analyzing affinities of libraries of binding compounds

摘要

A method of analysing affinities of a library of binding compounds to one or more ligands is disclosed comprising the steps of reacting one or more ligands with a library of binding compounds (e.g. antibodies), each binding compound consisting of or being encoded by a nucleotide sequence, determining the nucleotide sequences of binding compounds forming complexes with the ligands and of binding compounds free of ligand and determining the affinities of the respective binding compounds by comparing the number of times a nucleotide sequence is identified among binding compounds forming complexes with the ligands and the number of times the same nucleotide sequence is identified among the binding compounds free of the ligand. The library may be a phage display system expressing an antibody. Methods of identifying binding compounds with increased stability utilising the aforementioned steps are also claimed.